Coding

Part:BBa_K3819002:Design

Designed by: Luo SongWen   Group: iGEM21_UESTC-China   (2021-09-25)


Balac


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We have adopted strategies such as codon optimization to change the restriction site in order to be compatible with RFC.


Source

Gene ID: JN559771.1

References

[1]Kittl R, Mueangtoom K, Gonaus C, Khazaneh ST, Sygmund C, Haltrich D, Ludwig R. A chloride tolerant laccase from the plant pathogen ascomycete Botrytis aclada expressed at high levels in Pichia pastoris. J Biotechnol. 2012 Jan 20;157(2):304-14. doi: 10.1016/j.jbiotec.2011.11.021. Epub 2011 Dec 9. PMID: 22178779.